Research.CellAdhesion History

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August 11, 2010, at 04:13 PM by 130.126.231.65 -
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Our work spans length scales ranging from atomistic simulations to cell adhesion and migration in engineered environments. At the atomic level, steered molecular dynamics simulations identify structural features that are central to the mechanical functions of these proteins.  Sensitive, molecular level force-measurement techniques experimentally test predictions of the simulations, and investigate relationships between the structures of adhesion proteins, the mechanism and strengths of binding, and how these proteins respond to mechanical force. Measurements of binding kinetics between single cell pairs further test relationships betwen the properties of single protein bonds to the dynamics and strength of cell adhesion. 
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Our work spans length scales ranging from atomistic simulations to cell adhesion and migration in engineered environments. At the atomic level, steered molecular dynamics simulations identify structural features that are central to the mechanical functions of these proteins.  Sensitive, molecular level force-measurement techniques experimentally test predictions of the simulations, and investigate relationships between the structures of adhesion proteins, their mechanisms and strengths of binding, and their response to mechanical force. Measurements of binding kinetics between single cell pairs then test, at the cell level, relationships betwen the properties of single protein bonds to the dynamics and strength of cell adhesion. 
August 11, 2010, at 04:12 PM by 130.126.231.65 -
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%lfloat height=170px%http://www.scs.uiuc.edu/~leckband/uploads/Research/CellPolarization.jpg|Polarized cells on gradients.
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%lfloat height=200px%http://www.scs.uiuc.edu/~leckband/uploads/Research/CellPolarization.jpg|Polarized cells on gradients.
August 11, 2010, at 04:11 PM by 130.126.231.65 -
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%lfloat height=150px%http://www.scs.uiuc.edu/~leckband/uploads/Research/CellPolarization.jpg|Polarized cells on gradients.
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%lfloat height=170px%http://www.scs.uiuc.edu/~leckband/uploads/Research/CellPolarization.jpg|Polarized cells on gradients.
August 11, 2010, at 04:11 PM by 130.126.231.65 -
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More recent studies are now using live cell imaging in conjunction with patterned surface features to visualize directly how these signals trigger real-time spatiotemporal changes in cells as they encounter specific adhesive cues.  This powerful combination of precision surface engineering with live cell imaging will identify the biological design rules that define how cells read their environment to instruct cell behavior. 
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More recent studies use live cell imaging in conjunction with patterned surface features to visualize directly how these signals trigger real-time spatiotemporal changes in cells as they encounter specific cues.  This powerful combination of precision surface engineering with live cell imaging will identify crucial biological design rules that define how cells read their environment to instruct cell behavior. 
August 11, 2010, at 04:10 PM by 130.126.231.65 -
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More recent studies are now using live cell imaging in conjunction with patterned surface features to visualize directly how these signals trigger real-time spatiotemporal changes in cells as they encounter specific adhesive cues.  This powerful combination of precision surface engineering with live cell imaging will identify the biological design rules that define how cells read their environment to instruct cell behavior. 
August 11, 2010, at 04:04 PM by 130.126.231.65 -
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August 11, 2010, at 04:04 PM by 130.126.231.65 -
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Measurements of binding kinetics between single cell pairs are used to link binding properties of proteins measured at the single molecule level to the dynamics and strength of cell adhesion.  Our work spans length scales ranging from atomistic simulations to cell adhesion and migration in engineered environments.  At the atomic level, steered molecular dynamics simulations identify structural features that are central to the mechanical functions of these proteins.  Sensitive, molecular level force-measurement techniques experimentally test predictions of the simulations, and investigate relationships between the structures of adhesion proteins, the mechanism and strengths of binding, and how these proteins respond to mechanical force.
to:
Our work spans length scales ranging from atomistic simulations to cell adhesion and migration in engineered environments. At the atomic level, steered molecular dynamics simulations identify structural features that are central to the mechanical functions of these proteins.  Sensitive, molecular level force-measurement techniques experimentally test predictions of the simulations, and investigate relationships between the structures of adhesion proteins, the mechanism and strengths of binding, and how these proteins respond to mechanical force. Measurements of binding kinetics between single cell pairs further test relationships betwen the properties of single protein bonds to the dynamics and strength of cell adhesion. 
August 11, 2010, at 04:01 PM by 130.126.231.65 -
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Measurements of binding kinetics between single cell pairs are used to link binding properties of proteins measured at the single molecule level to the dynamics and strength of cell adhesion.  Sensitive force-measuring techniques are used to identify novel relationships between the structures of adhesion proteins, the mechanism and strengths of binding, and how they respond to mechanical force.
to:
Measurements of binding kinetics between single cell pairs are used to link binding properties of proteins measured at the single molecule level to the dynamics and strength of cell adhesion.  Our work spans length scales ranging from atomistic simulations to cell adhesion and migration in engineered environments.  At the atomic level, steered molecular dynamics simulations identify structural features that are central to the mechanical functions of these proteins.  Sensitive, molecular level force-measurement techniques experimentally test predictions of the simulations, and investigate relationships between the structures of adhesion proteins, the mechanism and strengths of binding, and how these proteins respond to mechanical force.
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We also use microfabrication and surface chemistry to generate controlled, concentration fields of adhesive cues, in order to determine how these signals direct cell migration or alter such cell functions as differentiation or proliferation.  Nanopatterned materials are used to ask whether cells sense nanoscale features as well as the size and spatial distribution of defined cues.  How many molecules are required to trigger cell signaling?  On what length scales do cells sense biochemical or mechanical differences?
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To determine how adhesive cues control cell functions, we use microfabrication and surface chemistry to generate controlled, concentration fields of adhesive cues, in order to determine how these signals direct cell migration or alter such cell functions as differentiation or proliferation.  Nanopatterned materials are used to ask whether cells sense nanoscale features as well as the size and spatial distribution of defined cues.  How many molecules are required to trigger cell signaling?  On what length scales do cells sense biochemical or mechanical differences?
August 11, 2010, at 03:57 PM by 130.126.231.65 -
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We are investigating molecular mechanisms of cell adhesion as well as the impact of adhesion on critical cell functions such as migration and signaling. 
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Research in cell adhesion focuses on molecular mechanisms of cell adhesion and on the impact of adhesion on critical cell functions such as migration and signaling. 
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August 11, 2010, at 03:55 PM by 130.126.231.65 -
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We also use microfabrication and surface chemistry to generate controlled, concentration fields of adhesive cues, in order to determine how these signals direct cell migration or alter such cell functions as differentiation or proliferation.  We can determine how cells sense nanoscale features as well as the size and spatial distribution of defined cues.
to:
We also use microfabrication and surface chemistry to generate controlled, concentration fields of adhesive cues, in order to determine how these signals direct cell migration or alter such cell functions as differentiation or proliferation.  Nanopatterned materials are used to ask whether cells sense nanoscale features as well as the size and spatial distribution of defined cues.  How many molecules are required to trigger cell signaling?  On what length scales do cells sense biochemical or mechanical differences?
August 11, 2010, at 03:53 PM by 130.126.231.65 -
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%rfloat height=100px%http://www.scs.uiuc.edu/~leckband/uploads/Research/MPA3.jpg
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%rfloat height=150px%http://www.scs.uiuc.edu/~leckband/uploads/Research/MPA3.jpg
August 11, 2010, at 03:52 PM by 130.126.231.65 -
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We also use microfabrication and surface modification to determine how concentration profiles of adhesive cues guides cell migration and controls such cell functions as differentiation and cell signaling.
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We also use microfabrication and surface chemistry to generate controlled, concentration fields of adhesive cues, in order to determine how these signals direct cell migration or alter such cell functions as differentiation or proliferation.  We can determine how cells sense nanoscale features as well as the size and spatial distribution of defined cues.
August 11, 2010, at 03:48 PM by 130.126.231.65 -
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 We also use microfabrication and surface modification to determine how concentration profiles of adhesive cues guides cell migration and controls such cell functions as differentiation and cell signaling.
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We also use microfabrication and surface modification to determine how concentration profiles of adhesive cues guides cell migration and controls such cell functions as differentiation and cell signaling.
August 11, 2010, at 03:48 PM by 130.126.231.65 -
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%lfloat height=150px%http://www.scs.uiuc.edu/~leckband/uploads/Research/CellPolarization.jpg|Cell polarization
on protein
gradients  We also use microfabrication and surface modification to determine how concentration profiles of adhesive cues guides cell migration and controls such cell functions as differentiation and cell signaling.
to:
%lfloat height=150px%http://www.scs.uiuc.edu/~leckband/uploads/Research/CellPolarization.jpg|Polarized cells on gradients.
 We also use microfabrication and surface modification to determine how concentration profiles of adhesive cues guides cell migration and controls such cell functions as differentiation and cell signaling.
August 11, 2010, at 03:47 PM by 130.126.231.65 -
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We also use microfabrication and surface modification to determine how concentration profiles of adhesive cues guides cell migration and controls such cell functions as differentiation and cell signaling.%lfloat height=150px%http://www.scs.uiuc.edu/~leckband/uploads/Research/CellPolarization.jpg
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%lfloat height=150px%http://www.scs.uiuc.edu/~leckband/uploads/Research/CellPolarization.jpg|Cell polarization
on protein gradients  We also use microfabrication and surface modification to determine how concentration profiles of adhesive cues guides
cell migration and controls such cell functions as differentiation and cell signaling.
August 11, 2010, at 03:46 PM by 130.126.231.65 -
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Measurements of binding kinetics between single cell pairs are used to link binding properties of proteins measured at the single molecule level to the dynamics and strength of cell adhesion. %rfloat height=100px%http://www.scs.uiuc.edu/~leckband/uploads/Research/MPA3.jpg  Sensitive force-measuring techniques are used to identify novel relationships between the structures of adhesion proteins, the mechanism and strengths of binding, and how they respond to mechanical force.
to:
%rfloat height=100px%http://www.scs.uiuc.edu/~leckband/uploads/Research/MPA3.jpg

Measurements
of binding kinetics between single cell pairs are used to link binding properties of proteins measured at the single molecule level to the dynamics and strength of cell adhesion.  Sensitive force-measuring techniques are used to identify novel relationships between the structures of adhesion proteins, the mechanism and strengths of binding, and how they respond to mechanical force.
August 11, 2010, at 03:45 PM by 130.126.231.65 -
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Measurements of binding kinetics between single cell pairs are used to link binding properties of proteins measured at the single molecule level to the dynamics and strength of cell adhesion. %rfloat height=100px%http://www.scs.uiuc.edu/~leckband/uploads/Research/MPA3.jpg.  Sensitive force-measuring techniques are used to identify novel relationships between the structures of adhesion proteins, the mechanism and strengths of binding, and how they respond to mechanical force.

We also use microfabrication and surface modification to determine how concentration profiles of adhesive cues guides cell migration and controls such cell functions as differentiation and cell signaling.%lfloat height=150px%http://www.scs.uiuc.edu/~leckband/uploads/Research/CellPolarization.jpg
to:
Measurements of binding kinetics between single cell pairs are used to link binding properties of proteins measured at the single molecule level to the dynamics and strength of cell adhesion. %rfloat height=100px%http://www.scs.uiuc.edu/~leckband/uploads/Research/MPA3.jpg  Sensitive force-measuring techniques are used to identify novel relationships between the structures of adhesion proteins, the mechanism and strengths of binding, and how they respond to mechanical force.


We also use microfabrication and surface modification to determine how concentration profiles of adhesive cues guides cell migration and controls such cell functions as differentiation and cell signaling.%lfloat height=150px%http://www.scs.uiuc.edu/~leckband/uploads/Research/CellPolarization.jpg








August 11, 2010, at 03:45 PM by 130.126.231.65 -
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We also use microfabrication and surface modification to determine how concentration profiles of adhesive cues guides cell migration and controls such cell functions as differentiation and cell signaling.%lfloat height=150px%www.scs.uiuc.edu/~leckband/uploads/Research/CellPolarization.jpg
to:
We also use microfabrication and surface modification to determine how concentration profiles of adhesive cues guides cell migration and controls such cell functions as differentiation and cell signaling.%lfloat height=150px%http://www.scs.uiuc.edu/~leckband/uploads/Research/CellPolarization.jpg
August 11, 2010, at 03:43 PM by 130.126.231.65 -
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Measurements of binding kinetics between single cell pairs are used to link binding properties at the single molecule level to the dynamics and strength of cell adhesion. %rfloat height=100px%http://www.scs.uiuc.edu/~leckband/uploads/Research/MPA3.jpg.  Sensitive force-measuring techniques determine relationships between protein architecture and the nanomechanics of protein-ligand bonds. 

Using microfabrication tools
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Measurements of binding kinetics between single cell pairs are used to link binding properties of proteins measured at the single molecule level to the dynamics and strength of cell adhesion. %rfloat height=100px%http://www.scs.uiuc.edu/~leckband/uploads/Research/MPA3.jpg.  Sensitive force-measuring techniques are used to identify novel relationships between the structures of adhesion proteins, the mechanism and strengths of binding, and how they respond to mechanical force.

We also use microfabrication and surface modification to determine how concentration profiles of adhesive cues guides cell migration and controls such cell functions as differentiation and cell signaling.%lfloat height=150px%www.scs.uiuc.edu/~leckband/uploads/Research/CellPolarization.jpg 
August 11, 2010, at 03:38 PM by 130.126.231.65 -
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Measurements of binding kinetics between single cell pairs are used to link binding properties at the single molecule level to the dynamics and strength of cell adhesion. %rfloat height=100px%http://www.scs.uiuc.edu/~leckband/uploads/Research/MPA3.jpg

and molecular modeling.
Sensitive force-measuring techniques determine relationships between protein architecture and the nanomechanics of protein-ligand bonds.  These biophysical approaches together with protein engineering identified key force transduction elements in protein structures. We are also establishing how the biophysical properties of individual proteins determine the strength and dynamics of adhesion between living cells. These approaches are used to determine molecular pathways that link adhesion proteins to the cytoskeleton and intracellular signaling.
to:
Measurements of binding kinetics between single cell pairs are used to link binding properties at the single molecule level to the dynamics and strength of cell adhesion. %rfloat height=100px%http://www.scs.uiuc.edu/~leckband/uploads/Research/MPA3.jpg. Sensitive force-measuring techniques determine relationships between protein architecture and the nanomechanics of protein-ligand bonds. 

Using microfabrication tools
August 11, 2010, at 03:36 PM by 130.126.231.65 -
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Measurements of binding kinetics between single cell pairs are used to link binding properties at the single molecule level to the dynamics and strength of cell adhesion. %rfloat height=100px%http://www.scs.uiuc.edu/~leckband/uploads/Research/MPA.jpg
to:
Measurements of binding kinetics between single cell pairs are used to link binding properties at the single molecule level to the dynamics and strength of cell adhesion. %rfloat height=100px%http://www.scs.uiuc.edu/~leckband/uploads/Research/MPA3.jpg
August 11, 2010, at 03:36 PM by 130.126.231.65 -
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Tissue development and tumor metastasis are two critical processes in which cell adhesion plays a central role. Cell adhesion provides mechanical linkages to other cells or to matrix, and many adhesion proteins are signaling molecules that transmit mechanical information to the cytosol. Conversely, adhesion dysfunction is causally linked to several diseases such as cancer. How cells (and adhesion proteins) sense and respond to these mechanical signals is an important, but still very young area of biology.

To address this topic, we use cell mechanical measurements, protein nanomechanics, biomolecular engineering,
and molecular modeling. Sensitive force-measuring techniques determine relationships between protein architecture and the nanomechanics of protein-ligand bonds.  These biophysical approaches together with protein engineering identified key force transduction elements in protein structures. We are also establishing how the biophysical properties of individual proteins determine the strength and dynamics of adhesion between living cells. These approaches are used to determine molecular pathways that link adhesion proteins to the cytoskeleton and intracellular signaling.
to:
We are investigating molecular mechanisms of cell adhesion as well as the impact of adhesion on critical cell functions such as migration and signaling. 

Measurements of binding kinetics between single cell pairs
are used to link binding properties at the single molecule level to the dynamics and strength of cell adhesion. %rfloat height=100px%http://www.scs.uiuc.edu/~leckband/uploads/Research/MPA.jpg

and molecular modeling. Sensitive force-measuring techniques determine relationships between protein architecture and the nanomechanics of protein-ligand bonds.  These biophysical approaches together with protein engineering identified key force transduction elements in protein structures. We are also establishing how the biophysical properties of individual proteins determine the strength and dynamics of adhesion between living cells. These approaches are used to determine molecular pathways that link adhesion proteins to the cytoskeleton and intracellular signaling.

Page last modified on August 11, 2010, at 04:13 PM