60

Burkhart, B.J.; Kakkar, N.; Hudson, G.A.; van der Donk, W.A.; Mitchell, D.A. "Chimeric Leader Peptides for the Generation of Non-Natural Hybrid RiPP Products" ACS Cent. Sci., Article ASAP (2017). doi:10.1021/acscentsci.7b00141

A “chimeric leader peptide” strategy enables combination of different RiPP enzymes to rationally design novel hybrid posttranslationally modified peptides.

59

Blin, K.; Wolf, T.; Chevrette, M.G.; Lu,X.; Schwalen, C.J.; Kautsar, S.A.; Suarez Duran, H.G.; de los Santos, E.L.C.; Kim, H.U.; Nave, M.; Dickschat, J.S.; Mitchell, D.A.; Shelest, E.; Breitling, R.; Takano, E.; Lee, S.Y.; Weber, T.; Medema, M. "antiSMASH 4.0 - improvements inchemistry prediction and gene cluster boundary identification" Nucleic Acids Res., gkx319 (2017). https://doi.org/10.1093/nar/gkx319

The latest version of antiSMASH, the premier natural products genome-mining tool, is updated with RODEO's RiPP detection algorithms.

58

Schwalen, C.J.; Feng, X.; Liu, W.; O-Dowd, B.; Ko, T.P.; Shin, C.J.; Guo, R.T.; Mitchell, D.A.; Oldfield, E. ""Head-to-head" Prenyl Synthases in Some Pathogenic Bacteria." ChemBioChem, Accepted Article (2017). doi:10.1002/cbic.201700099

Genome mining of pathogens was used to guide enzymatic characterization of new prenyltransferases.

57

Mahanta, N.; Zhang, Z.; Hudson, G.A.; van der Donk, W.; Mitchell, D.A. "Reconstitution and substrate specificity of the radical SAM thiazole C-methyltransferase in thiomuracin biosynthesis" J. Am. Chem. Soc., 139: 4310–4313 (2017). doi:10.1021/jacs.7b00693

Characterization of the substrate selectivity and regioselectivity of TbtI, a radical SAM methyltransferase that acts upon an unactivated sp2 thiazole carbon.

56

Burkhart, B.J.; Schwalen, C.J.; Mann, G.; Naismith, J.H.; Mitchell, D.A. "YcaO-Dependent Posttranslational Amide Activation: Biosynthesis, Structure, and Function." Chem. Rev., 117: 5389-5456 (2017). doi:10.1021/acs.chemrev.6b00623

A review of all biosynthetic pathways with a YcaO, the rules governing cyclodehydratases, and the possible role of YcaOs in thioamide and amidine formation.

55

Tietz, J.I.; Schwalen, C.J.; Patel, P.S.; Maxson, T.; Blair, P.M.; Tai, H.C.; Zakai, U.Z.; Mitchell, D.A. "A new genome mining tool redefines the lasso peptide biosynthetic landscape." Nat. Chem. Biol., 13:470-478 (2017). doi:10.1038/nchembio.2319

Design of a new genome mining tool guides mapping of a RiPP family and discovery of several new antimicrobial natural products.

54

Maxson, T.; Tietz, J.I.; Hudson, G.A.; Guo, X.R.; Tai, H.; Mitchell, D.A. "Targeting reactive carbonyls for identifying natural products and their biosynthetic origins." J. Am. Chem. Soc., 138: 15157-15166 (2016). doi:10.1021/jacs.6b06848

Development and use of a new probe for reactivity-based screening to discover a novel natural product and its biosynthetic origin.

53

Zhang, Z.; Hudson, G.A.; Mahanta, N.; Tietz, J.I.; van der Donk, W.A.; Mitchell, D.A. "Biosynthetic timing and substrate specificity for the thiopeptide thiomuracin." J. Am. Chem. Soc., 138: 15511–15514 (2016). doi:10.1021/jacs.6b08987

A study on the substrate specificity and order of modifications in the biosynthesis of thiomuracin.

52

Deane, C.D.; Burkhart, B.J.; Blair, P.M.; Tietz, J.I.; Lin, A.; Mitchell, D.A. "In vitro biosynthesis and substrate tolerance of the plantazolicin family of natural products." ACS Chem. Biol., 11: 2232−2243 (2016). doi:10.1021/acschembio.6b00369

Characterization of the synthetase and new natural products from the plantazolicin family.

51

Molloy, E.M.; Tietz, J.I.; Blair, P.M.; Mitchell, D.A. "Biological characterization of the hygrobafilomycin antibiotic JBIR-100 and bioinformatic insights into the hygrolide family of natural products." Bioorg. Med. Chem., 24: 6276-6290 (2016). doi:10.1016/j.bmc.2016.05.021

Genomics and reactivity-based labeling were used to identify a hygrobafilomycin gene cluster, probe bioactivity, and elucidate structure.

50

Tietz, J.I.; Mitchell, D.A. "Using genomics for natural product structure elucidation." Curr. Top. Med. Chem., 16:1645-1694 (2016). doi:10.2174/1568026616666151012111439

A review discussing the use of genomic information to discover and elucidate or confirm the structure of novel natural products.

49

Molohon, K.; Blair, P.; Park, S.; Doroghazi, J.R.; Maxson, T.; Hershfield, J.; Flatt, K.; Schroeder, N.; Ha, T.; Mitchell, D.A. "Plantazolicin is an ultra-narrow spectrum antibiotic that targets the Bacillus anthracis membrane." ACS Infect. Dis., 2:207-220 (2016). doi:10.1021/acsinfecdis.5b00115

The scope of bioactivity and action of plantazolicin on the bacteria cell membrane is elucidated.

48

Hudson, G.A.; Zhang, Z.; Tietz, J.I.; Mitchell, D.A.; van der Donk, W.A. "In vitro biosynthesis of the core scaffold of the thiopeptide thiomuracin." J. Am. Chem. Soc., 137:16012-16015 (2015). doi:10.1021/jacs.5b10194

The total enzymatic synthesis of a thiomuracin antibiotic.

47

Maxson, T.; Bertke, J.A.; Gray, D.L.; Mitchell, D.A. "Crystal structure and absolute configuration of (3S,4aS,8aS)-N-tert-butyl-2-[(S)-3-(2-chloro-4-nitrobenzamido)-2-hydroxy-propyl]decahydroisoquinoline-3-carboxamide and (3S,4aS,8aS)-N-tert-butyl-2-{(S)-2-[(S)-1-(2-chloro-4-nitrobenzoyl)pyrrolidin-2-yl]-2-hydroxyethyl}decahydroisoquinoline-3-carboxamide." Acta Cryst., E17:1401-1407 (2015). doi:10.1107/S2056989015020046

Description of the crystal structures for two nelfinavir analogs, establishing the absolute configuration and intra- and inter-molecular interactions.

46

Cox, C.L.; Doroghazi, J.R.; Mitchell, D.A. "The genomic landscape of ribosomal peptides containing thiazole and oxazole heterocycles." BMC Genomics, 16:778 (2015). doi:10.1186/s12864-015-2008-0

A comprehensive mining effort reveals the genomic landscape of linear azol(in)e-containing peptide biosynthetic gene clusters.

45

Maxson, T.; Mitchell, D.A. "Targeted treatment for bacterial infections: Prospects for pathogen-specific antibiotics coupled with rapid diagnostics." Tetrahedron (Special Issue on Natural Product-Inspired Approaches to Combat Bacteria), 72: 3609-3624 (2016). doi:10.1016/j.tet.2015.09.069

A review discussing the benefits and drawbacks of narrow-spectrum antibiotics and the diagnostics needed to employ them.

44

Medema, M.H.; et al. "Minimum Information about a Biosynthetic Gene cluster." Nat. Chem. Biol., 11:625-631 (2015). doi:10.1038/nchembio.1890

The MIBIG specification and database provide a community standard for description and annotation of biosynthetic gene clusters.

43

Molloy, E.M.; Casjens, S.R.; Cox, C.L.; Maxson, T.; Ethridge, N.A.; Margos, G.; Fingerle, V.; Mitchell, D.A. "Identification of the minimal cytolytic unit for streptolysin S and an expansion of the toxin family." BMC Microbiol., 15:141 (2015). doi:10.1186/s12866-015-0464-y

Newly-identified truncated SLS-like precursor peptides facilitate a greater understanding of SLS structure-activity relationship.

42

Burkhart, B.J.; Hudson, G.A.; Dunbar, K.L.; Mitchell, D.A. "A prevalent peptide-binding domain guides ribosomal natural product biosynthesis" Nat. Chem. Biol., 11:564-570 (2015). doi:10.1038/nchembio.1856

A conserved peptide binding domain recruits precursor peptides to enzymes in diverse RiPP biosynthetic pathways.

41

Dunbar, K.L.; Tietz, J.I.; Cox, C.L.; Burkhart, B.J.; Mitchell, D.A. "Identification of an auxiliary leader peptide-binding protein required for azoline formation in ribosomal natural products" J. Am. Chem. Soc., 137:7672-7677 (2015). doi:10.1021/jacs.5b04682

A novel linear azol(in)e-containing peptide biosynthetic protein is shown to be involved in substrate binding and cyclodehydratase activation.

40

Maxson, T.; Deane, C.D.; Molloy, E.M.; Cox, C.L.; Markley, A.L.; Lee, S.W.; Mitchell, D.A. "HIV protease inhibitors block streptolysin S production" ACS Chem. Biol., 10:1217–1226 (2015). doi:10.1021/cb500843r

An HIV protease inhibitor is repurposed to block the production of the virulence factor streptolysin S from S. pyogenes.

39

Hao, Y.; Blair, P.M.; Sharma, A.; Mitchell, D.A.; Nair, S.K. "Insights into methyltransferase specificity and bioactivity of derivatives of the antibiotic plantazolicin" ACS Chem. Biol., 10:1209–1216 (2015). doi:10.1021/cb501042a

Plantazolicin substructures are used to probe methyltransferase activity and antibacterial specificity.

38

Metelev, M.; Tietz, J.I.; Melby, J.O.; Blair, P.M.; Zhu, L.; Livnat, I.; Severinov, K.; Mitchell, D.A. "Structure, bioactivity, and resistance mechanism of streptomonomicin, an unusual lasso peptide from an understudied halophilic actinomycete" Chem. Biol., 22:241-250 (2015). doi:10.1016/j.chembiol.2014.11.017

An unusual lasso peptide antibiotic is characterized, and genome sequencing predicts biosynthetic potential in an overlooked genus.

37

Dunbar, K.L.; Chekan, J.R.; Cox, C.L.; Burkhart, B.J.; Nair, S.K.; Mitchell, D.A. "Discovery of a new ATP-binding motif involved in peptidic azoline biosynthesis" Nat. Chem. Biol., 10:823-829 (2014). doi:10.1038/nchembio.1608

X-ray structure of a YcaO family member resolves the linear azol(in)e-containing peptide cyclodehydratase ATP-binding pocket and active site.

36

Zhang, Q.; Ortega, M; Shi, Y.; Wang, H.; Melby, J.O.; Tang, W.; Mitchell, D.A.; van der Donk, W.A. "Structural investigation of ribosomally synthesized natural products by hypothetical structure enumeration and evaluation using tandem MS" Proc. Natl. Acad. Sci. USA, 111:12031-12036 (2014). doi:10.1073/pnas.1406418111

An algorithm-based method uses tandem mass spectra for automatic RiPP structure assignment.

35

Cox, C.L.; Tietz, J.I.; Sokolowski, K.; Melby, J.O.; Doroghazi, J.R.; Mitchell, D.A. "Nucleophilic 1,4-additions for natural product discovery" ACS Chem. Biol., 9:2014-2022 (2014). doi:10.1021/cb500324n

Bioinformatics-prioritized reactivity-based screening identifies a new thiopeptide antibiotic.

34

Sinko, W.; Wang, Y.; Zhu, W.; Zhang, Y.; Feixas, F.; Cox, C.; Mitchell, D.A.; Oldfield, E.; McCammon, J.A. "Undecaprenyl diphosphate synthase inhibitors: antibacterial drug leads" J. Med. Chem., 57:5693–5701 (2014). doi:10.1021/jm5004649

Computer-identified inhibitors of undecaprenyl disphosphate synthase, a novel target in cell wall biosynthesis, kill drug-resistant pathogens.

33

Li, K.; Schurig-Briccio, L.A.; Feng, X.; Upadhyay, A.; Pujari, V.; Lechartier, B.; Fontes, F.L.; Yang, H.; Rao, G.; Zhu, W.; Gulati, A.; No, J.H.; Cintra, G.; Bogue, S.; Liu, Y.-L.; Molohon, K.; Orlean, P.; Mitchell, D.A.; Freitas-Junior, L.; Ren, F.; Sun, H.; Jiang, T.; Li, Y.; Guo, R.-T.; Cole, S.T.; Gennis, R.B.; Crick, D.C.; Oldfield, E. "Multitarget drug discovery for tuberculosis and other infectious diseases." J. Med. Chem., 57:3126-3139 (2014). doi:10.1021/jm500131s

Establishment of antibacterial, antifungal, and antimalarial SAR, as well as mammalian cell toxicity, for a panel of M. tuberculosis drug analogs.

32

Deane, C.D.; Mitchell, D.A. "Lessons learned from the transformation of natural product discovery to a genome-driven endeavor." J. Ind. Microbiol. Biot., 41:315-331 (2014). doi:10.1007/s10295-013-1361-8

A review on "reverse" discovery of natural products, including lessons learned and recommendations for the future of the field.

31

Melby, J.O.; Li, X.; Mitchell, D.A. "Orchestration of enzymatic processing by thiazole/oxazole-modified microcin dehydrogenases." Biochemistry, 53:413-422 (2014). doi:10.1021/bi401529y

By separating cyclodehydratase and dehydrogenase activity, dehydrogenase promiscuity and selectivity was investigated.

30

Sharma, A.; Blair, P.M.; and Mitchell, D.A. "Synthesis of plantazolicin analogues enables dissection of ligand binding interactions of a highly selective methyltransferase." Org. Lett., 15:5076-5079 (2013). doi:10.1021/ol402444a

PZN truncations were synthesized to study binding requirements of its methyltransferase.

29

Lee, J.; Hao, Y.; Blair, P.M.; Melby, J.O.; Agarwal, V.; Burkhart, B.J.; Nair, S.K.; Mitchell, D.A. "Structural and functional insight into an unexpectedly selective N-methyltransferase involved in plantazolicin biosynthesis." Proc. Natl. Acad. Sci. USA, 110:12954-12959 (2013). doi:10.1073/pnas.1306101110

Studies in the biosynthesis of plantazolicin reveal a methyltransferase with unprecedented selectivity for its substrate.

28

Deane, C.D.; Melby, J.O.; Molohon, K.J.; Susarrey, A.R.; Mitchell, D.A. "Engineering unnatural variants of plantazolicin through codon reprogramming." ACS Chem. Biol., 8:1998-2008 (2013). doi:10.1021/cb4003392

Mutagenesis of the PZN precursor highlights the selectivity of its biosynthetic enyzmes.

27

Dunbar, K.L.; Mitchell, D.A. "Insights into the mechanism of peptide cyclodehydrations achieved through the chemoenzymatic generation of amide derivatives." J. Am. Chem. Soc., 135:8692-9701 (2013). doi:10.1021/ja4029507

The linear azol(in)e-containing peptide cyclodehydratase was used to install isotope labels into peptide backbones, and the resultant product was used as a mechanistic probe.

26

Hu, Y.; Jia, S.; Ren, F.; Huang, C.-H.; Ko, T.-P.; Mitchell, D.A.; Guo, R.-T.; Zheng, Y. "Crystallization and preliminary X-ray diffraction of YisP protein from Bacillus subtilis subsp. subtilis strain 168," Acta Cryst., F69:77-79 (2013). doi:10.1107/S1744309112049330

Crystals for structural studies of recombinant B. subtilis protein YisP were obtained, allowing insight into isoprenoid biosynthesis in this organism.

25

Dunbar, K.L.; Mitchell, D.A. "Revealing Nature's synthetic potential through the study of ribosomal natural product biosynthesis." ACS Chem. Biol., 8:473-487 (2013). doi:10.1021/cb3005325

A review focusing on the diverse biological chemistry discovered in the study of ribosomal natural product biosynthetic enzymes.

24

Zhu, W.; Zhang, Y.; Sinko, W.; Hensler, M.E.; Olson, J.; Molohon, K.J.; Lindert, S.; Cao, R.; Li, K.; Wang, K.; Wang, Y.; Liu, Y.-L.; Sankovsky, A.; de Oliveira, C.A.F.; Mitchell, D.A.; Nizet, V.; McCammon, J.A.; Oldfield, E. "Antibacterial drug leads targeting isoprenoid biosynthesis." Proc. Natl. Acad. Sci. USA, 110:123-128 (2013). doi:10.1073/pnas.1219899110

X-ray structures of ten antibacterial compounds reveal binding to the undecaprenyl diphosphate synthase, an essential cell wall biosynthesis enzyme.

23

Arnison, P., et al. "Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature." Nat. Prod. Rep., 30:108-160 (2013). doi:10.1039/C2NP20085F

A comprehensive review on ribosomal natural products with a systematic naming system presented for the research community.

22

Zhang, Y.; Lin, F.-Y.; Li, K.; Zhu, W.; Liu, Y.-L.; Cao, R.; Pang, R.; Lee, E.; Axelson, J.; Hensler, M.; Wang, K.; Molohon, K.J.; Wang, Y.; Mitchell, D.A.; Nizet, V.; Oldfield, V. “HIV-1 integrase inhibitor-inspired antibacterials targeting isoprenoid biosynthesis.” ACS Med. Chem. Lett., 3:402-406 (2012). doi:10.1021/ml300038t

Possessing a similar motif to HIV-1 integrase, prenyl transferases UPPS and CrtM are targeted by keto and diketo-acid compounds.

21

Dunbar, K.L.; Melby, J.O.; Mitchell, D.A. "YcaO domains use ATP to activate amide backbones during peptide cyclodehydrations." Nat. Chem. Biol., 8:569-575 (2012). doi:10.1038/nchembio.944

A biochemically novel mechanism for ATP use is demonstrated in the context of linear azol(in)e-containing peptide cyclodehydratases.

20

Melby, J.O.; Dunbar, K.L.; Trinh, N.Q.: Mitchell, D.A. "Selectivity, directionality, and promiscuity in peptide processing from a Bacillus sp. Al Hakam cyclodehydratase." J. Am. Chem. Soc., 134:5309-5316 (2012). doi:10.1021/ja211675n

The substrate processing of a Bacillus sp. Al Hakam cyclodehydratase was assessed using mass spectrometry and kinetics.

19

Molohon, K.J.; Melby, J.O.; Lee, J.; Evans, B.S.; Dunbar, K.L.; Bumpus, S.B.; Kelleher, N.L.; Mitchell, D.A. "Structure determination and interception of biosynthetic intermediates for the plantazolicin class of highly discriminating antibiotics." ACS Chem. Biol., 6:1307-1313 (2011). doi:10.1021/cb200339d

The structure of plantazolicin, a B. anthracis-specific antibiotic, is solved by MS and NMR.

18

Molloy, E.; Cotter, P.D.; Hill, C.; Mitchell, D.A.; Ross, R.P. “Streptolysin S-like virulence factors: the continuing SagA.” Nat. Rev. Microbiol., 9:670-681 (2011). doi:10.1038/nrmicro2624

Review of the genetics, biochemistry, and biological functions of Streptolysin S, a virulence-associated cytolytic linear azol(in)e-containing peptide produced by Streptococcus pyogenes.

17

Pei, J.; Mitchell, D.A.; Dixon, J.E.; Grishin, N.V. "Expansion of type II CAAX proteases reveals evolutionary origin of γ-secretase subunit APH-1." J Mol. Biol., 410:18-26 (2011). doi:10.1016/j.jmb.2011.04.066

The discovery that the predicted protease within many linear azol(in)e-containing peptide clusters shares ancestry with a seemingly unrelated eukaryotic protease, gamma-secretase.

16

Melby, J.O.; Nard, N.J.; Mitchell, D.A. "Thiazole/oxazole-modified microcins: Complex natural products from ribosomal templates." Curr. Op. Chem. Biol., 15:369-378 (2011). doi:10.1016/j.cbpa.2011.02.027

Review of the linear azol(in)e-containing peptide natural product family with an emphasis on the evolution of novel natural products.

15

Scholz, R.; Molohon, K.J.; Nachtigall, J.; Vater, J.; Markley, A.L.; Sussmuth, R.D.; Mitchell, D.A.; Borriss, R. “Plantazolicin, a novel microcin B17/streptolysin S-like natural product from Bacillus amyloliquefaciens FZB42.” J. Bacteriol., 193:215-224 (2011). doi:10.1128/JB.00784-10

Discovery of plantazolicin, a posttranslationally modified metabolite which shows specific antimicrobial activity.

14

Gonzalez, D.J.; Lee, S.W.; Hensler, M.E.; Dahesh, S.; Markley, A.L.; Mitchell, D.A.; Banderia, N.; Nizet, V.; Dixon, J.E.; Dorrestein, P.C. “Clostridiolysin S: a post-translationally modified biotoxin from Clostridium botulinum.” J. Biol. Chem., 285:28220-28228 (2010). doi:10.1074/jbc.M110.118554

13

Mitchell, D.A.; Ryabov, A.D.; Kundu, S.; Chanda, A,; Collins, T.J. "Oxidation of pinacyanol chloride by H2O2 catalyzed by FeIII complexed to tetraamidomacrocyclic ligand: unusual kinetics and product identification." J. Coord. Chem., 63:2605-2618 (2010). doi:10.1080/00958972.2010.492426

12

Haft, D.; Basu, M.; Mitchell, D.A. “Expansion of ribosomally produced natural products: a nitrile hydratase- and Nif11-related precursor family.” BMC Biol., 8:70 (2010). doi:10.1186/1741-7007-8-70

A bioinformatics-based discovery of novel linear azol(in)e-containing peptide natural products with uncharacteristically long leader peptides that derive from known enzymes.

11

Mitchell, D.A.; Lee, S.W.; Pence, M.A.; Markley, A.L.; Limm, J.D.; Nizet, V.; Dixon, J.E. “Structural and functional dissection of the heterocyclic peptide cytotoxin streptolysin S.” J. Biol. Chem., 284:13004-13012 (2009). doi:10.1074/jbc.M900802200

Identification of residues for synthetase binding and cytolytic activity for the streptolysin S precursor peptide.

10

Ghosh, A.; Mitchell, D.A.; Chanda, A.; Ryabov, A.D.; Popescu, D.L.; Upham, E.; Collins, T.J. “Catalase-peroxidase activity of iron(III)-TAML activators of hydrogen peroxide.” J. Am. Chem. Soc., 130:15116-15126 (2008). doi:10.1021/ja8043689

9

Lee, S.W.; Mitchell, D.A.; Markley, A.L.; Hensler, M.E.; Gonzalez, D.; Wohlrab, A.; Dorrestein, P.C.; Nizet, V.; Dixon, J.E. “Discovery of a widely distributed toxin biosynthetic gene cluster.” Proc. Natl. Acad. Sci. USA, 105: 5879-5884 (2008). doi:10.1073/pnas.0801338105

The in vitro reconstitution of streptolysin S biosynthesis and the bioinformatics-based discovery of the linear azol(in)e-containing peptide natural product family.

8

Mitchell, D.A.; Morton, S.U.; Fernhoff, N.B.; Marletta, M.A. “Thioredoxin is required for S-nitrosation of procaspase-3 and inhibits apoptosis in Jurkat cells.” Proc. Natl. Acad. Sci. USA, 104:11609-11614 (2007). doi:10.1073/pnas.0704898104

7

Mitchell, D.A., Marletta, M.A., Michel, T. “Effects of S-nitrosation of nitric oxide synthase isoforms.” (Review) Adv. Exp. Biol., Nitric Oxide 1, Ch. 7. Elsevier Publishing

6

Chahbane, N.; Popescu, D.L.; Mitchell, D.A.; Chanda, A.; Lenoir, D.; Ryabov, A.D.; Schramm, K.W.; Collins, T.J., “Fe(III)-TAML catalyzed green oxidative degradation of the azo dye Orange II by H2O2 and organic peroxides: products, toxicity, kinetics, and mechanisms.” Green Chem., 9:1-10 (2007). doi:10.1039/B604990G

5

Mitchell, D.A.; Morton, S.U.; Marletta, M.A. “Design and characterization of an active site selective caspase-3 transnitrosating agent.” ACS Chem. Biol., 1:659-665 (2006). doi:10.1021/cb600393x

4

Erwin, P.A.; Mitchell, D.A.; Sartoretto, J.; Marletta, M.A.; Michel, T. “Subcellular targeting and differential S-nitrosylation of endothelial nitric oxide synthase.” J. Biol. Chem., 281:151-157 (2006). doi:10.1074/jbc.M510421200

3

Mitchell, D.A.; Marletta, M.A., “Thioredoxin catalyzes the S-nitrosation of the caspase-3 active site cysteine.” Nat. Chem. Biol., 1:154-158 (2005). doi:10.1038/nchembio720

2

Mitchell, D.A.; Erwin, P.A.; Michel, T.; Marletta, M.A., “S-Nitrosation and regulation of inducible nitric oxide synthase.” Biochemistry, 44:4636-4647 (2005). doi:10.1021/bi0474463

1

Lazo, J.S.; Nemoto, K.; Pestell, K.E.; Cooley, K.; Southwick, E.C.; Mitchell, D.A.; Furey, W.; Gussio, R.; Zaharevitz, D.W.; Joo, B.; Wipf, P., “Identification of a potent and selective pharmacophore for Cdc25 dual specificity phosphatase inhibitors.” Mol. Pharmacol., 61:720-728 (2002). doi:10.1124/mol.61.4.720