RIENSTRA GROUP
 
UNIVERSITY OF ILLINOIS
 
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Deborah Berthold

Staff Scientist

Office: A129C CLSL
Phone: (217) 333-7606
E-mail: berthold_at_illinois.edu
 
 

education

Ph.D. Plant Biochemistry, Duke University
B.S. Biochemistry, B.A. Philosophy, Michigan State University
 

research interests

Deborah Berthold is a senior research scientist whose main interests concern the structure and function of membrane proteins, especially those that contain a quinone binding site. Her long-term research project involves the alternative oxidase, a respiratory enzyme containing a carboxylate-bridged diiron center at its active site. The alternative oxidase is found in plants as well as the human pathogens, Trypanosoma sp. and Cryptosporidium parvum. Currently Dr. Berthold's research is focused determining the structure of the transmembrane protein DsbB by SSNMR. This quinone reductase is one component of a pathway that is responsible for forming disulfide bonds in proteins of the E. coli periplasm. Deborah has developed new and improved methods for efficient expression and purification of isotopically labeled membrane proteins while learning NMR data acquisition and analysis techniques.

 

selected publications

"Structure of the Disulfide Bond Generating Membrane Protein DsbB in the Lipid Bilayer", Tang M, Nesbitt AE, Sperling LJ, Berthold DA, Schwieters CD, Gennis RB, Rienstra CM. J. Mol. Biol., 2013, 425, 1670-1682 (Published online, Feb 14, 2013).

"Chemical shift assignment of the transmembrane helices of DsbB, a 20-kDa integral membrane enzyme, by 3D magic-angle spinning NMR spectroscopy", Li, Y.; Berthold, D. A.; Gennis, R. B.; Rienstra, C. M. Protein Sci. (Accelerated Publication) 2008, 17, 199-204. (Published online, February 1, 2008).

"Partial 13C and 15N chemical shift assignments of the disulfide bond forming enzyme DsbB by 3D magic-angle spinning NMR spectroscopy", Li, Y.; Berthold, D. A.; Frericks, H. L.; Gennis, R. B.; Rienstra, C. M., ChemBioChem 2007, 8, 434-442. (Published online February 7, 2007).

"Membrane-bound dirron carboxylate proteins", Berthold, D. A.; Stenmark, P., Annu. Rev. Plant Biol. 2003, 54, 497-517. (Published online June 2003).

"EPR studies of the mitochrondrial alternative oxidase: Evidence for a diiron carboxylate center", Berthold, D. A.; Voevodskaya, M.; Stenmark, P.; Graeslund, A.; Nordlund, P., J. Biol. Chem. 2002, 277, 43608-43614. (Published online September 4, 2002).

"New insight into the structure and function of the alternative oxidase", Berthold, D. A.; Andersson, M. E.; Nordlund, P., Biochim. Biophys. Acta 2000 1460, 241-254. (Available online November 20, 2000).

"Isolation of mutants of the Arabidopsis thaliana alternative oxidase (ubiquinol:oxygen oxidoreductase) resistant to salicylhydroxamic acid", Berthold, D. A., Biochim. Biophys. Acta 1998, 1364, 73-83. (Available online October 16, 1998).

"Partial purification of the cyanide-resistant alternative oxidase of skunk cabbage (Symplocarpus foetidus) mitochondria", Berthold, D. A.; Siedow, J. N., Plant Physiol. 1993, 101, 113-119. (Available online).

"Determination of molecular mass of the aroid alternative oxidase by radiation inactivation analysis", Berthold, D. A.; Fluke, D. J.; Siedow, J. N., Biochem. J. 1988, 252, 73-77. (Available online).

"A highly resolved, oxygen-evolving photosystem II preparation from spinach thylakoid membranes: EPR and electron-transport properties", Berthold, D. A.; Babcock, G. T.; Yocum, C. F., Febs Lett. 1981, 134, 231-234. (Available online).