RIENSTRA GROUP
 
UNIVERSITY OF ILLINOIS
 
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Sample PreparatioN

CIL Application Note #15:

"Top Ten Tips for producing 13C 15N protein in abundance"

Deborah A. Berthold, Victoria J. Jeisy, Terry L. Sasser, John J. Shea, Heather L. Frericks, Gautam Shah, and Chad M. Rienstra

10. Stop the leaks.
9. Slow down the train.
8. Eat yer spinach.
7. Be media-savvy.
6. Exercise *aerobically*.
5. Timing is everything. (part i: Induction)
4. Timing is everything (part II: Harvest)
3. Quadruple up on the cells.
2. Just a spponful of sugar (or four).
1. Save both the baby and the bathwater.