LAB SYLLABUS, SPRING 2001: SECTION A

 

EXPERIMENT

LAB DATES

DUE DATE*

RETURN DATE

BOOK

 

Check-in/Excel tutorial

 

1/17

 

¾

 

¾

 

¾

 

Photometry

 

1/19

 

1/24

 

1/26

 

I

 

Dipeptide analysis

 

1/22,24,26

 

1/31

 

2/2

 

II

 

Microanalysis of CHO

 

1/29,31; 2/2

 

2/7

 

2/9

 

I

 

Erythrocyte membranes

 

2/5,7,9

 

2/14

 

2/19

 

II

 

Properties of b-gal

 

 

2/12,14,16,19

 

2/26

 

3/2

 

I

EXAM I :  Tuesday, February 20 (7-9 PM)                  TOPICS:  Photometry – Membranes

 

Column chromatography

 

2/21

 

2/26

 

3/2

 

I

 

GOT purification/

SDS-PAGE

 

2/23,26,28;

3/2,5,7

 

 

3/21

 

 

3/26

 

 

II

 

GST purif. & Western blot

 

 

3/9

 

¾

 

¾

 

I

SPRING BREAK

3/10-18

 

 

 

 

GST purif. & Western blot

 

3/19,21

 

3/26

 

3/30

 

I

 

ELISA

 

3/23,26,28

 

4/2

 

4/4

 

II

 

Design lab†

 

3/30

 

4/13

 

4/20

 

¾

 

Kinases

 

 

4/2,4

 

4/9

 

4/13

 

I

EXAM II :  Thursday, April 5 (7-9 PM)                                            TOPICS:  b-gal – ELISA

 

Recombinant plasmids

 

4/6,9,11,13

 

4/18

 

4/23

 

II

 

PCR

 

4/16,18

 

4/25

 

4/30

 

I

 

Transcription

 

4/20,23

 

4/25

 

4/30

 

I

 

Lab practical

 

4/25,27

 

5/4

 

~5/9

 

II

 

Computer lab

 

 

4/30; 5/1

 

5/4

 

~5/9

 

¾

EXAM III :  Tuesday, May 1 (7-9 PM)                             TOPICS:  Kinases – Transcription

 

Check-out  (Last lab day)

 

5/2

 

¾

 

¾

 

¾

 

*LAB REPORTS ARE DUE AT 1:00 PM MONDAY-FRIDAY ON THE DATES INDICATED.

†LAB REPORT TO BE COMPLETED ON NOTEBOOK PAPER, SEPARATE FROM LAB BOOKS.

 

ALL EXAMS WILL BE HELD IN 116 ROGER ADAMS LAB.